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KMID : 1144820180240010030
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대한의생명과학회지
2018 Volume.24 No. 1 p.30 ~ p.34
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Effect of the Ag3PO4 on Staphylococcus aureus Growth and Human Immunity
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Kim Mi-Kyung
Kim Dae-Sik
Kim Dae-Sik
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Abstract
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Silver (Ag) has been widely used in commercial products and medical fields since ancient times because of its antibacterial effect. It is harmless and non-toxic to the human body. For this reason, recent research has actively evaluated antimicrobial activity using silver (Ag). In this study, we investigated the inhibitory effect of a silver-based compound, silver phosphate (Ag3PO4) on the growth of Staphylococcus aureus and the activation of human immunity. First, the inhibitory effect of Ag3PO4 on the growth of Staphylococcus aureus was confirmed by a growth curve and a colony-ounting method. As a result, the growth inhibitory effect increased as the concentration of Ag3PO4 increased. Specifically, treatment with 5 μg/mL of Ag3PO4 resulted in no bacteria growth, and the colony-counting method showed a remarkable inhibition. In addition, the expression of cytokine IL-8 by Ag3PO4 was examined to investigate the cellular immune system activation by Ag3PO4. After pretreatment of Staphylococcus aureus for 1 hour with 50 μg/mL Ag3PO4, an increased IL-8 mRNA expression resulted. In cells treated with Ag3PO4, we found that the expression of IL-8 was enhanced in a time-dependent fashion compared to non-treated cells. These results indicate that Ag3PO4 induces antimicrobial activity against Staphylococcus aureus and activates human immunity. These results are expected to contribute to the future study of the mechanism of silver (Ag) and silver-based compounds in relation to antibacterial activity.
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KEYWORD
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Silver, Staphylococcus aureus, Antibacterial, Immunity
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